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chef-drii pfge apparatus  (Bio-Rad)


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    Bio-Rad chef-drii pfge apparatus
    Chef Drii Pfge Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chef-drii+pfge+apparatus/chef+drii+pfge+apparatus/pmc11077071-94-5-8
    Average 90 stars, based on 1 article reviews
    chef-drii pfge apparatus - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Lambda DNA Preparation:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Pulsed-Field Gel:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Electrophoresis:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Molecular Weight:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Marker:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.

    Staining:

    Article Title: Telomere-Internal Double-Strand Breaks Are Repaired by Homologous Recombination and PARP1/Lig3-Dependent End-Joining
    Article Snippet: Digested DNA was resolved on a 1% agarose/0.5XTBE gel using a CHEF-DRII PFGE apparatus (Bio-Rad) for 24 hr.

    Article Title: The Streptomycin-Sulfadiazine-Tetracycline Antimicrobial Resistance Element of Calf-Adapted Escherichia coli Is Widely Distributed among Isolates from Washington State Cattle
    Article Snippet: PFGE was performed on a CHEF-DRII PFGE apparatus (Bio-Rad) using a 1% agarose gel (Seakem Gold-agarose; FMC BioProducts) in 0.5× Tris-borate-EDTA at 14°C and 6 V/cm, with an initial switch time of 2.2 s and a final switch time of 54.2 s. Gels were run for 18 h and were stained with ethidium bromide for UV illumination.

    Article Title: Causes of Pneumonia Epizootics among Bighorn Sheep, Western United States, 2008–2010
    Article Snippet: Isolates were subjected to pulsed-field gel electrophoresis (PFGE) performed on a CHEF-DRII PFGE apparatus (Bio-Rad, Hercules, CA, USA) in 1% agarose gel (Seakem Gold Agarose; FMC Bio Products, Rockland, MD, USA) in 0.5× Tris borate EDTA buffer at 14 ° C for 20 h at 6 V/cm and a linear ramp of 1.0–30.0 s for ApaI or 0.5–40.0 s for Sma I. Salmonella serovar Braenderup H9812, digested with Xba I for 3 h at 37 ° C, was used as a size standard on each gel.

    Article Title: pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus
    Article Snippet: Slices of embedded cells were then loaded into the wells of a 1% agarose gel, 0.5X TBE (1X TBE = 0.49 M Tris-HCl, 0.49 M boric acid, 0.001 M EDTA; pH8), and run in a BioRad CHEF-DRII PFGE apparatus at 14°C and 6 v/cm for 19 hrs, with a switch time beginning at 15s and ramping to 75s.

    Article Title: DNA-PK controls Apollo’s access to leading-end telomeres
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V/cm at 14°C.

    Article Title: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres.
    Article Snippet: DNA was resolved by a CHEF-DRII PFGE apparatus (Bio-Rad) for 20 h, with the following settings: initial pulse, 5 s; final pulse, 5 s; 6 V cm−1 at 14 °C.

    Article Title: TRF2 binds branched DNA to safeguard telomere integrity.
    Article Snippet: The six-subunit shelterin complex (composed of TRF1, TRF2, Rap1, TIN2, TPP1 and POT1)1 is required to prevent telomeres from being recognized as sites of DNA damage2,3.. Within shelterin, TRF2 is involved in the repression of two end-initiated DNA-damageresponse pathways: the ATM kinase signaling pathway and nonhomologous end joining (NHEJ)4–7.. TRF2 prevents ATM signaling and NHEJ by sequestering the chromosome end in the t-loop structure8,9.

    Article Title: A Shld1-controlled POT1a provides support for repression of ATR signaling at telomeres through RPA exclusion
    Article Snippet: Plugs were loaded on a 1% agarose/0.5 × TBE gel and run for 24 h using CHEF-DRII PFGE apparatus (BioRad) in 0.5 × TBE running buffer.



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